Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody diluted at 1:2000.Application
NBS1 antibody immunoprecipitates nibrin protein in IP experiments. IP samples: NT2D1 whole cell extract A. 50 μg NT2D1 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of nibrin protein by 4 μg NBS1 antibody 7.5 % SDS-PAGE The immunoprecipitated nibrin protein was detected by NBS1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
NBS1 antibody detects NBS1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NBS1 protein stained by NBS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody diluted at 1:2000.Application
NBS1 antibody detects NBS1 protein by western blot analysis. A. 30 μg NT2D1 whole cell extract 7.5 % SDS-PAGE NBS1 antibody dilution: 1:5000| Product Name | NBS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NBS1 (nibrin)Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NBS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AT-V1 nibrin NBN P95 AT-V2 ATV NBS |
|---|---|
| Target | NBS1 |
| Molecular Weight(MW) | 85 kDa (note) (2) |
| Cellular Localization | Nucleus (By similarity) , Telomere (By similarity) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody diluted at 1:2000.
Application
NBS1 antibody immunoprecipitates nibrin protein in IP experiments. IP samples: NT2D1 whole cell extract A. 50 μg NT2D1 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of nibrin protein by 4 μg NBS1 antibody 7.5 % SDS-PAGE The immunoprecipitated nibrin protein was detected by NBS1 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
NBS1 antibody detects NBS1 protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NBS1 protein stained by NBS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody diluted at 1:2000.
Application
NBS1 antibody detects NBS1 protein by western blot analysis. A. 30 μg NT2D1 whole cell extract 7.5 % SDS-PAGE NBS1 antibody dilution: 1:5000| Positive Control | 293T , NT2D1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
