Application
N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using N-Cadherin antibody antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | N-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to N-Cadherin (cadherin 2, type 1, N-cadherin (neuronal))This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human N-Cadherin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Chicken, BovineDog (100%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | neural-cadherin antibody neural cadherin antibody calcium-dependent adhesion protein neuronal antibody cadherin-2 antibody cadherin 2 type 1 N-cadherin (neuronal) antibody cadherin 2 N-cadherin (neuronal) antibody NCAD antibody N-cadherin |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (2) |
| Cellular Localization | Cell membrane |
Application
N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin protein stained by N-Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using N-Cadherin antibody antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , HepG2 , Mouse brain , Rat brain , *A549 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
