Application
N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 5% SDS-PAGE N-Cadherin antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using N-Cadherin antibody at 1:500 dilution.Application
N-Cadherin antibody [N1N3] detects CDH2 protein at membrane on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: K562 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin protein stained by N-Cadherin antibody [N1N3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | N-Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to N-Cadherin (cadherin 2, type 1, N-cadherin (neuronal))This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human N-Cadherin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Chicken, BovineXenopus laevis (82%), Dog (96%), Chicken (90%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | neural-cadherin antibody neural cadherin antibody calcium-dependent adhesion protein neuronal antibody cadherin-2 antibody cadherin 2 type 1 N-cadherin (neuronal) antibody cadherin 2 N-cadherin (neuronal) antibody NCAD antibody N-cadherin |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (2) |
| Cellular Localization | Cell membrane |
Application
N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis. A. 30 µg PC-12 whole cell extract B. 30 µg Rat2 whole cell extract 5% SDS-PAGE N-Cadherin antibody [N1N3] dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using N-Cadherin antibody at 1:500 dilution.
Application
N-Cadherin antibody [N1N3] detects CDH2 protein at membrane on hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded human hepatoma. N-Cadherin antibody [N1N3] dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: K562 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin protein stained by N-Cadherin antibody [N1N3] diluted at 1:250. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | K562 , mouse brain , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
