Application
Sample (20 ug of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:10000Application
Sample (5 ug of whole cell lysate) A: Rat brain 5% SDS PAGE diluted at 1:10000Application
NCAM antibody detects NCAM protein by western blot analysis. Human tissue extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NCAM antibody diluted at 1:10000.| Product Name | NCAM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCAM (neural cell adhesion molecule 1)This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NCAM. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Cat, Chicken, BovineDog (94%), Cat (96%), Chicken (87%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD56 antibody MSK39 antibody NCAM antibody NCAM1 antibody NCAM-1 antibody neural cell adhesion molecule 1 antibody N-CAM-1 antibody antigen recognized by monoclonal antibody 5.1H11 antibody "neural cell adhesion molecule NCAM antibody" |
|---|---|
| Molecular Weight(MW) | 95 kDa (note) (5) |
| Cellular Localization | Isoform 1: Cell membrane; Single-pass type I membrane protein , Isoform 2: Cell membrane; Single-pass type I membrane protein , Isoform 3: Cell membrane; Lipid-anchor , GPI-anchor , Isoform 4: Cell membrane; Lipid-anchor , Isoform 5: Secreted , Isoform 6: |
Application
Sample (20 ug of whole cell lysate) A: mouse brain 5% SDS PAGE diluted at 1:10000
Application
Sample (5 ug of whole cell lysate) A: Rat brain 5% SDS PAGE diluted at 1:10000
Application
NCAM antibody detects NCAM protein by western blot analysis. Human tissue extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NCAM antibody diluted at 1:10000.| Positive Control | Human brain , Mouse liver , Rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
