Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 10% SDS PAGE diluted at 1:1000| Product Name | NCF4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCF4 (neutrophil cytosolic factor 4, 40kDa)The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Full length human NCF4 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse, Rat, RabbitBovine (89%), Mouse (86%), Rat (87%), Rabbit (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC3810 antibody NCF antibody P40PHOX antibody SH3PXD4 antibody NCF4 antibody p40-phox antibody neutrophil NADPH oxidase factor 4 antibody neutrophil cytosol factor 4 antibody NCF-4 antibody SH3 and PX domain-containing protein 4 antibody "neutro |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 10% SDS PAGE diluted at 1:1000| Positive Control | Jurkat , Raji , K562 , THP-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
