Rabbit Polyclonal antibody to Nck beta (NCK adaptor protein 2)This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human Nck beta. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Xenopus laevis, Pig, Chicken, BovineMouse (95%), Rat (95%), Xenopus laevis (83%), Pig (90%), Chicken (92%), Bovine (90%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
GRB4 antibody NCKbeta antibody NCK2 antibody SH2/SH3 adaptor protein NCK-beta antibody "noncatalytic region of tyrosine kinase beta antibody" cytoplasmic protein NCK2 antibody NCK adaptor protein 2 antibody
Molecular Weight(MW)
43 kDa (note) (2)
Cellular Localization
Cytoplasm , Endoplasmic reticulum
Application
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:10000
Positive Control
A549 , HepG2
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.