Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using NCOA62 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:10000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NCOA62 antibody at 1:500 dilution.| Product Name | NCOA62 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCOA62 (SNW domain containing 1)This gene, a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also function as a splicing factor by interacting with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NCOA62. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Chimpanzee, BovineRat (98%), Zebrafish (80%), Xenopus laevis (83%), Chimpanzee (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bx42 antibody MGC119379 antibody NCOA-62 antibody PRPF45 antibody Prp45 antibody SKIIP antibody SKIP antibody SNW1 antibody SKI-interacting protein antibody nuclear receptor coactivator NCoA-62 antibody SKI interacting protein antibody "nuclear |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using NCOA62 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE diluted at 1:10000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NCOA62 antibody at 1:500 dilution.| Positive Control | A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
