Rabbit Polyclonal antibody to ND5A small number of complex I subunits are the products of mitochondrial genes (subunits 1-7), while the remainder are nuclear encoded and imported from the cytoplasm. NADH dehydrogenase subunit 5 (ND5) is most likely a component of the hydrophobic protein fragment of Complex I. Mutations in the gene encodiing for ND5 are implicated in Mitochondrial neurogastrointestinal encephalomyopathy, an autosomal recessive multisystem disorder that results in dramatically elevated levels of circulating thymidine and deoxyuridine.
Immunogen
Synthetic peptide, corresponding to amino acids 321-370 of Human ND5.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
NADH dehydrogenase subunit 5 (complex I) MTND5
Target
ND5
Tissue Specificity
ND5 (M359) pAb detects endogenous levels of ND5 protein.
Application
Application
Western blot (WB) analysis of ND5 (M359) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate
Application Notes
WB: 1:500 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Storage Buffer
supplied in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.