Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using NDUFA10 antibody at 1:250 dilution.Application
NDUFA10 antibody detects NDUFA10 protein by Western blot analysis. A. 50 µg mouse kidney lysate/extract 10% SDS-PAGE NDUFA10 antibody dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed A431, using NDUFA10 antibody at 1:200 dilution.| Product Name | NDUFA10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFA10 (NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa)The protein encoded by this gene belongs to the complex I 42kDA subunit family. Mammalian complex I is the first enzyme complex in the electron transport chain of mitochondria. It is composed of 45 different subunits. This protein is a component of the hydrophobic protein fraction and has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDUFA10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (99%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CI-42KD antibody CI-42k antibody FLJ21923 antibody MGC5103 antibody NDUFA10 antibody complex I 42kDa subunit antibody "NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10 mitochondrial antibody" NADH-ubiquinone oxidoreductase 42 kDa subun |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) (3) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using NDUFA10 antibody at 1:250 dilution.
Application
NDUFA10 antibody detects NDUFA10 protein by Western blot analysis. A. 50 µg mouse kidney lysate/extract 10% SDS-PAGE NDUFA10 antibody dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed A431, using NDUFA10 antibody at 1:200 dilution.| Positive Control | Jurkat , mouse kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
