Application
Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.Application
NDUFB4 antibody detects NDUFB4 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. NDUFB4 antibody dilution: 1:500.Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.| Product Name | NDUFB4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFB4 (NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa)The protein encoded by this gene belongs to the complex I NDUFB4 subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq] |
| Immunogen | Full length human NDUFB4 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B15 antibody CI-B15 antibody MGC5105 antibody NDUFB4 antibody NADH-ubiquinone oxidoreductase B15 subunit antibody complex I B15 subunit antibody complex I-B15 antibody NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 antibody "NADH dehyd |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane; Single-pass membrane protein; Matrix side |
Application
Sample (30 ug of whole cell lysate) A: HepG2 15% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFB4 antibody at 1:500 dilution.
Application
NDUFB4 antibody detects NDUFB4 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. NDUFB4 antibody dilution: 1:500.
Application
Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using NDUFB4 antibody at 1:500 dilution.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
