Application
NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted by 1:1000.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NDUFS3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: THP-1 B: HL-60 12% SDS PAGE diluted at 1:1000| Product Name | NDUFS3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFS3 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial)This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDUFS3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (92%), Rat (91%), Zebrafish (81%), Xenopus laevis (83%), Pig (93%), Chicken (90%), Rhesus Monkey (98%), Chimpanzee (96%), Bovine (94%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CI-30 antibody NDUFS3 antibody complex I 30kDa subunit antibody NADH dehydrogenase-ubiquinone 30 kDa subunit antibody CI-30kD antibody NADH-ubiquinone oxidoreductase 30 kDa subunit antibody "NADH dehydrogenase [ubiquinone] iron-sulfur protein 3 mito |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (2) |
| Cellular Localization | Mitochondrion inner membrane |
Application
NDUFS3 antibody detects NDUFS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NDUFS3 antibody diluted by 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using NDUFS3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: THP-1 B: HL-60 12% SDS PAGE diluted at 1:1000| Positive Control | THP-1 , HL-60 , Jurkat , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
