Application
Immunofluorescence analysis of methanol-fixed HeLa, using NDUFS4 antibody at 1:100 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NDUFS4 antibody detects NDUFS4 protein by western blot analysis. A. 50 µg Rat heart lysate/extract 12% SDS-PAGE NDUFS4 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NDUFS4 antibody at 1:100 dilution.Application
Sample(30 µg whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NDUFS4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NDUFS4 (NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Q reductase))This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NDUFS4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (99%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AQDQ antibody CI-18 antibody NDUFS4 antibody CI-AQDQ antibody CI-18 kDa antibody "NADH-coenzyme Q reductase 18-KD antibody" mitochondrial respiratory chain complex I (18-KD subunit) antibody complex I 18kDa subunit antibody complex I-AQDQ antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein; Matrix side |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using NDUFS4 antibody at 1:100 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NDUFS4 antibody detects NDUFS4 protein by western blot analysis. A. 50 µg Rat heart lysate/extract 12% SDS-PAGE NDUFS4 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NDUFS4 antibody at 1:100 dilution.
Application
Sample(30 µg whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , mouse brain , rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
