Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PVRL1 antibody diluted at 1:500.| Product Name | Nectin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nectin 1 (poliovirus receptor-related 1 (herpesvirus entry mediator C))This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Nectin 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, PigMouse (90%), Rat (89%), Dog (95%), Pig (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PVRR PRR OFC7 PVRR1 poliovirus receptor-related 1 (herpesvirus entry mediator C) SK-12 HIgR nectin-1 ED4 HVEC CLPED1 PRR1 C |
|---|---|
| Target | Nectin 1 |
| Molecular Weight(MW) | 57 kDa (note) (3) |
| Cellular Localization | Isoform Alpha: Cell membrane; Single-pass type I membrane protein , Isoform Delta: Cell membrane; Single-pass type I membrane protein , Isoform Gamma: Secreted |
Application
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PVRL1 antibody diluted at 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
