Application
FACS analysis of THP-1 cells using Nectin 1 antibody (green) or an isotype control (black).Application
ICC/IF analysis of PC12 cells using Nectin 1 antibody at a dilution of 1:100 (red). (B) Live image of the cells. (C) Merge of the two images.Application
IHC-Fr analysis of rat cerebellum using Nectin 1 antibody at a dilution of 1:100 (red) and DAPI (blue). (C) Merge of the two images.Application
WB analysis of Jurkat (lanes 1 and 3) and MEG-01 (lanes 2 and 4) lysates using Nectin 1 antibody at a dilution of 1:500 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.| Product Name | Nectin 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nectin 1This gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobulin superfamily and has 3 extracellular immunoglobulin-like loops, a single transmembrane domain (in some isoforms), and a cytoplasmic region. This protein acts as a receptor for glycoprotein D (gD) of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), and pseudorabies virus (PRV) and mediates viral entry into epithelial and neuronal cells. Mutations in this gene cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini. [provided by RefSeq, Oct 2009] |
| Immunogen | Peptide (C)GKPPSVVSWETRLK, corresponding to amino acid residues 177- 190 of human nectin-1 (Accession Q15223). Extracellular, N-terminus. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHCWB |
| WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PVRR HIgR PVRL1 OFC7 CD111 PRR poliovirus receptor-related 1 (herpesvirus entry mediator C) SK-12 PVRR1 ED4 HVEC nectin-1 P |
|---|---|
| Target | Nectin 1 |
Application
FACS analysis of THP-1 cells using Nectin 1 antibody (green) or an isotype control (black).
Application
ICC/IF analysis of PC12 cells using Nectin 1 antibody at a dilution of 1:100 (red). (B) Live image of the cells. (C) Merge of the two images.
Application
IHC-Fr analysis of rat cerebellum using Nectin 1 antibody at a dilution of 1:100 (red) and DAPI (blue). (C) Merge of the two images.
Application
WB analysis of Jurkat (lanes 1 and 3) and MEG-01 (lanes 2 and 4) lysates using Nectin 1 antibody at a dilution of 1:500 in the absence (lens 1 and 2) or presence (lens 3 and 4) of blocking peptide.| Application Notes | WB:1:500~1:1000 ICC/IF:1:100~1:500 IHC:1:50~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA and 0.05% NaN3. |
