Application
Immunoprecipitation of Neutrophil elastase protein from HepG2 whole cell extracts using 5 μg of Neutrophil elastase antibody [N2C3] Neutrop . Western blot analysis was performed using Neutrophil elastase antibody [N2C3] Neutrop . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: A549 B: HepG2 12% SDS PAGE diluted at 1:1000| Product Name | Neutrophil elastase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Elastase (Neutrophil ) (elastase, neutrophil expressed)Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Elastase 2 hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix following the protein's release from activated neutrophils. Elastase 2 may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is clustered with other serine protease gene family members, azurocidin 1 and proteinase 3 genes, at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Neutrophil elastase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ELA2 antibody GE antibody HLE antibody HNE antibody NE antibody PMN-E antibody SCN1 antibody ELANE antibody leukocyte elastase antibody human leukocyte elastase antibody "elastase 2 neutrophil antibody" granulocyte-derived elastase antibody me |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
Application
Immunoprecipitation of Neutrophil elastase protein from HepG2 whole cell extracts using 5 μg of Neutrophil elastase antibody [N2C3] Neutrop . Western blot analysis was performed using Neutrophil elastase antibody [N2C3] Neutrop . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: A549 B: HepG2 12% SDS PAGE diluted at 1:1000| Positive Control | A549 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
