Application
NFIA antibody detects NFIA protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NFIA protein stained by NFIA antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
NFIA antibody detects NFIA protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIA protein stained by NFIA antibody diluted at 1:1000. Blue: Hoechst 33342 staining.Application
NFIA antibody detects NFIA protein by western blot analysis. A. 30 µg HeLa nuclear lysate/extract 10% SDS-PAGE NFIA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
NFIA antibody detects NFIA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NFIA antibody dilution: 1:500.Application
NFIA antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NFIA antibody dilution: 1:500.Application
NFIA antibody detects NFIA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. NFIA antibody dilution: 1:500.| Product Name | NFIA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFIA (nuclear factor I/A)Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NFIA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chicken, BovineChicken (98%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NF-I/A NF1-A NFI-L CTF nuclear factor I/A NFI-A |
|---|---|
| Target | NFIA |
| Molecular Weight(MW) | 56 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
NFIA antibody detects NFIA protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: NFIA protein stained by NFIA antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
NFIA antibody detects NFIA protein at nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIA protein stained by NFIA antibody diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
NFIA antibody detects NFIA protein by western blot analysis. A. 30 µg HeLa nuclear lysate/extract 10% SDS-PAGE NFIA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
NFIA antibody detects NFIA protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NFIA antibody dilution: 1:500.
Application
NFIA antibody detects NFIA protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. NFIA antibody dilution: 1:500.
Application
NFIA antibody detects NFIA protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. NFIA antibody dilution: 1:500.| Positive Control | HeLa nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
