Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF kappa B-p100/p52 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HT29 cells using NF kappa B-p100/p52 Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using NF kappa B-p100/p52 Antibody .| Product Name | NFkB p100 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p100NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. |
| Immunogen | Peptide sequence around aa.864~868 (E-D-S-A-Y) derived from Human NFkb p100. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LYT-10 LYT10 p52 NFKB2 nuclear factor NF-kappa-B p100 subunit nuclear factor of kappa light chain gene enhancer in B-cells 2 oncogene Lyt-10 DNA-binding factor KBF2 lymphocyte translocation chromosome 10 protein nuclear factor of kappa light polyp |
|---|---|
| Target | NFkB p100/p52 |
| Molecular Weight(MW) | 120 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF kappa B-p100/p52 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HT29 cells using NF kappa B-p100/p52 Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using NF kappa B-p100/p52 Antibody .| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
