Application
Immunofluorescence staining of methanol-fixed Hela cells using NF kappa B-p105/p50 (Phospho-Ser927) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF kappa B-p105/p50 (Phospho-Ser927) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HL60 cells untreated or treated with TNF- alpha using NF kappa B-p105/p50 (Phospho-Ser927) Antibody .Application
Western blot analysis of extracts from HL60 cells untreated or treated with TNF-a using NFkB-p105 (Phospho-Ser927) Antibody| Product Name | NFkB p105 (phospho Ser927) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NF kappa B-p105/p50 (Phospho Ser927)NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. |
| Immunogen | Peptide sequence around phosphorylation site of serine 927 (C-D-S(p)-G-V) derived from Human NF kappa B-p105. |
| Modification | p-Ser927 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686C01211 EBP-1 KBF1 MGC54151 NF-kappa-B NF-kappaB NFKB-p105 NFKB-p50 p105 p50 NFKB1 nuclear factor kappa-B DNA binding subunit NF-kappabeta nuclear factor NF-kappa-B p105 subunit DNA binding factor KBF1 nuclear factor NF-kappa-B p50 s |
|---|---|
| Target | NFkB p105 (phospho Ser927) |
| Molecular Weight(MW) | 50120 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using NF kappa B-p105/p50 (Phospho-Ser927) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NF kappa B-p105/p50 (Phospho-Ser927) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HL60 cells untreated or treated with TNF- alpha using NF kappa B-p105/p50 (Phospho-Ser927) Antibody .
Application
Western blot analysis of extracts from HL60 cells untreated or treated with TNF-a using NFkB-p105 (Phospho-Ser927) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
