Application
Western blot analysis of NF-kappaB p105/p50 (pS337) expression in HeLa (A); HEK293T TNF-treated (B); NIH3T3 (C); Raw264.7 (D); rat liver (E) whole cell lysates.Application
Immunohistochemical analysis of NF-kappaB p105/p50 (pS337) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | NFkB p105/p50 (phospho Ser337) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p105/p50 (phospho Ser337)This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p105/p50. The exact sequence is proprietary. |
| Modification | p-Ser337 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NF-kappa-B KBF1 p105 NF-kB1 NFKB-p105 EBP-1 p50 NFKB1 NF-kappaB NFKB-p50 nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 Ant |
|---|---|
| Target | NFkB p105/p50 (phospho Ser337) |
| Tissue Specificity | Recognizes endogenous levels of NF-kappaB p105/p50 (pS337) protein. |
Application
Western blot analysis of NF-kappaB p105/p50 (pS337) expression in HeLa (A); HEK293T TNF-treated (B); NIH3T3 (C); Raw264.7 (D); rat liver (E) whole cell lysates.
Application
Immunohistochemical analysis of NF-kappaB p105/p50 (pS337) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
