Application
Western blot of HeLa cell extract. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.| Product Name | NFkB p50 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p50The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the disregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis and cancer. |
| Immunogen | Synthetic peptide: AEDDPYLGRFEQMF conjugated to KLH. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAEMSAWB |
| WB:1:500~1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P50 NF KB1 NFKB1 P105 NFKB P105 EBP1 NF KAPPAB KBF1 NF-KAPPA B NFKB P50 NFKAPPAB |
|---|---|
| Target | NFkB p105/p50 |
| Cellular Localization | Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I kappa B). |
Application
Western blot of HeLa cell extract. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.| Application Notes | WB:1:500~1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.01% (w/v) Sodium Azide |
