Application
Western blot analysis of NF-kappaB p65 (AcK221) expression in HepG2 TSA-treated (A); mouse brain (B) whole cell lysates.| Product Name | NFkB p65 (Acetyl Lys221) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p65 (Acetyl Lys221)NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NF-kappaB p65 (AcK221). The exact sequence is proprietary. |
| Modification | Acetyl Lys221 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | v-rel reticuloendotheliosis viral oncogene homolog A (avian) p65 RELA NFKB3 |
|---|---|
| Target | NFkB p65 |
| Tissue Specificity | Recognizes endogenous levels of NF-kappaB p65 (AcK221) protein. |
Application
Western blot analysis of NF-kappaB p65 (AcK221) expression in HepG2 TSA-treated (A); mouse brain (B) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
