Application
WB analysis of HeLa lysates using NFkB p65 (phospho Ser276) antibody in the presence or absence of blocking peptide.Application
IHC-P analysis of human breast carcinoma tissue using NFkB p65 (phospho Ser276) antibody.Application
ICC/IF analysis of methanol-fixed HeLa cells using NFkB p65 (phospho Ser276) antibody (red) and DAPI (blue).| Product Name | NFkB p65 (phospho Ser276) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFkB p65 (phospho Ser276)NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding S276 of human RELA |
| Modification | p-Ser276 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:20 - 1:50: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RELA NFKB3 p65 v-rel reticuloendotheliosis viral oncogene homolog A (avian) |
|---|---|
| Target | NFkB p65 (phospho Ser276) |
| Molecular Weight(MW) | 60 KDa (note) |
Application
WB analysis of HeLa lysates using NFkB p65 (phospho Ser276) antibody in the presence or absence of blocking peptide.
Application
IHC-P analysis of human breast carcinoma tissue using NFkB p65 (phospho Ser276) antibody.
Application
ICC/IF analysis of methanol-fixed HeLa cells using NFkB p65 (phospho Ser276) antibody (red) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:100. IF: 1:20 - 1:50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
