Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary NFKB p65 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = hepatocarcinomaApplication
Western blot analysis of Phospho-NFKBp65-S536 Antibody in human TNF alpha activated Hela cell line lysates. Phospho-RPS6KB1 (arrow) was detected using the purified PAb. Lane 1: without TNF alpha Lane 2: 10ng/ml TNF alpha, 5min| Product Name | NFKB p65 (phospho Ser536) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NFKB p65 phospho S536NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] |
| Immunogen | Synthetic phosphopeptide corresponding to amino acid residues surrounding S536 of human NFKBp65. |
| Modification | p-Ser536 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIHC-PWB |
| For ELISA: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC131774 NFKB3 p65 NFKB p65 (phospho S536) NFKB p65 (phospho Ser536) NFKB p65 (pS536) NFKB p65 (pSer536) NFKB p65 phospho S536 NFKB p65 phospho Ser536 Phospho NFKB p65 (S536) Phospho NFKB p65 (Ser536) Phospho NFKB p65 (pS536) Phospho NFKB p65 |
|---|---|
| Target | NFKB p65 phospho S536 |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary NFKB p65 antibody , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. HC = hepatocarcinoma
Application
Western blot analysis of Phospho-NFKBp65-S536 Antibody in human TNF alpha activated Hela cell line lysates. Phospho-RPS6KB1 (arrow) was detected using the purified PAb. Lane 1: without TNF alpha Lane 2: 10ng/ml TNF alpha, 5min| Positive Control | TNF alpha activated Hela cell lysate, Hepatocarcinoma |
|---|---|
| Application Notes | For ELISA: Use at a dilution of 1:1,000. For IHC-P: Use at a dilution of 1:50-1:100. For WB: Use at a dilution of 1:100-1:500. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH7.2 containing 0.09% sodium azide |
