Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 7.5% SDS PAGE diluted at 1:10000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using 68kDa Neurofilament Light antibody at 1:500 dilution.| Product Name | NF-L antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to 68kDa Neurofilament Light (neurofilament, light polypeptide)Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human 68kDa Neurofilament Light. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CMT1F antibody CMT2E antibody FLJ53642 antibody NF-L antibody NF68 antibody NFL antibody NEFL antibody "neurofilament protein light chain antibody" neurofilament triplet L protein antibody "neurofilament light polypeptide 68kDa antibody" 68 kDa |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) |
Application
Sample (30 ug of whole cell lysate) A: PC-3 B: U87-MG C: SK-N-SH 7.5% SDS PAGE diluted at 1:10000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using 68kDa Neurofilament Light antibody at 1:500 dilution.| Positive Control | PC-3 , U87-MG , SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
