Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYA antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using NFYA antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | NFYA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFYA (nuclear transcription factor Y subunit alpha)The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq] |
| Immunogen | Full length human NFYA Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (97%), Rhesus Monkey (91%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CBF-A antibody CBF-B antibody FLJ11236 antibody HAP2 antibody NF-YA antibody NFYA antibody CAAT box DNA-binding protein subunit A antibody CCAAT-binding transcription factor subunit B antibody "Transcription factor NF-Y A subunit antibody" CAAT-b |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYA antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded D54 xenograft, using NFYA antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
