Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFYB antibody at 1:500 dilution.Application
Sample (20 ug ) A: HeLa 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYB antibody at 1:500 dilution.Application
NFYB antibody [N1C3-2] detects NFYB protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NFYB antibody [N1C3-2] diluted at 1:500.| Product Name | NFYB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NFYB (nuclear transcription factor Y, beta)The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Rhesus Monkey, BovineRat (98%), Xenopus laevis (91%), Pig (99%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CBF-A antibody CBF-B antibody HAP3 antibody NF-YB antibody NFYB antibody nuclear transcription factor Y subunit beta antibody "Transcription factor NF-Y B subunit antibody" CAAT box DNA-binding protein subunit B antibody CCAAT-binding transcriptio |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using NFYB antibody at 1:500 dilution.
Application
Sample (20 ug ) A: HeLa 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NFYB antibody at 1:500 dilution.
Application
NFYB antibody [N1C3-2] detects NFYB protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NFYB antibody [N1C3-2] diluted at 1:500.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
