Application
Western blot analysis of NIFK in 30 ugs of HeLa cell lysate using at 1:1000.Application
NIFK KI67ip -immunostaining in adipocytes of human carcinomatus breast tissue using at 1:500 dilution.| Product Name | NIFK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NIFKNIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent. |
| Immunogen | Synthetic peptides corresponding to amino acids 144-160 and 276-293 of human NIFK. Amino acids 144-160 share a 40% homology with mouse NIFK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB: Use at a dilution of 1/500 - 1/1000. GTX13912 detects NIFK in 30 μgs of HeLa cell lysate Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | NIFK Nopp34 |
|---|---|
| Target | NIFK |
Application
Western blot analysis of NIFK in 30 ugs of HeLa cell lysate using at 1:1000.
Application
NIFK KI67ip -immunostaining in adipocytes of human carcinomatus breast tissue using at 1:500 dilution.| Positive Control | HeLa whole cell lysate |
|---|---|
| Application Notes | WB: Use at a dilution of 1/500 - 1/1000. GTX13912 detects NIFK in 30 μgs of HeLa cell lysate Predicted molecular weight: 34 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide, 0.2% GelatinConstituents: PBS |
