Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NIP30 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 12% SDS PAGE diluted at 1:1000Application
NIP30 antibody detects NIP30 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NIP30 protein stained by NIP30 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | NIP30 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NIP30 (family with sequence similarity 192, member A) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NIP30. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (92%), Rat (86%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C16orf94 antibody CDA018 antibody FLJ21799 antibody MGC74898 antibody NIP30 antibody FAM192A antibody NEFA-interacting nuclear protein NIP30 antibody protein FAM192A antibody "family with sequence similarity 192 member A antibody" |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NIP30 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A549 B: H1299 12% SDS PAGE diluted at 1:1000
Application
NIP30 antibody detects NIP30 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NIP30 protein stained by NIP30 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | A549 , H1299 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
