Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NIPA antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Product Name | NIPA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NIPA (zinc finger, C3HC-type containing 1)Essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NIPA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (88%), Bovine (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 1110054L24Rik antibody FBXO50 antibody NIPA antibody ZC3HC1 antibody nuclear interaction partner of ALK antibody "zinc finger C3HC type 1 antibody" nuclear-interacting partner of anaplastic lymphoma kinase antibody nuclear interacting partner of an |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NIPA antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
