Application
NKX3-1 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of NKX3-1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab.| Product Name | NKX3-1 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NKX3-1 (Internal)The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.[supplied by OMIM] |
| Immunogen | KLH conjugated synthetic peptide between 124~154 amino acids from the Center region of human NKX3-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NKX31 NKX3.1 NKX3A NKX3-1 NKX3.1 NKX3A Homeobox protein NK3 homolog A Homeobox protein Nkx3.1 Homeobox protein NK-3 homolog A Homeobox protein Nkx-3.1 NKX31 Homeobox protein Nkx 3.1 |
|---|---|
| Target | NKX3-1 |
| Molecular Weight(MW) | 26.35 kDa (note) |
Application
NKX3-1 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of NKX3-1 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
