| Product Name | NKX3A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NKX3ANKX3A is a member of the NK3 homeobox family and has several isoforms. It is a putative transcription factor and may act as a transcriptional repressor that exhibits prostate and testis specific expression. It may play a role in proliferation of glandular epithelium and in formation of ducts in the prostate. NKX3A is regulated by androgens and estrogen in the prostate cancer cell line LNCaP. |
| Immunogen | Synthetic peptide: PRLPQTPKQPQKRS conjugated to BSA, corresponding to amino acids 70-83 of Human NKX3A, isoform v1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC: Use at a dilution of 1/200 - 1/1000. WB: Use at a dilution of 1/200 - 1/1000. Detects a band of approximately 21.6 - 26.3 kDa. Preparation InstructionsMethod 1 for immunostaining and immunoblotting (Western blot): Remove a fixed amount of suspension and dissolve 1/5 in PBS or TBS to yield a 0.1 mg/ml solution. Method 2 for immunoprecipitation, supershift, immunostaining and immunoblotting (Western blot): Pellet antibodies. Remove supernatant. Dissolve the pellet in 50ul of PBS (or TBS) at final concentration of 1 mg/ml. Do not allow the pellet to dry. Do not vortex. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAPX2 NKX3 NKX3.1 NKX3A |
|---|---|
| Target | NKX3A |
| Cellular Localization | Nuclear |
| Application Notes | IHC: Use at a dilution of 1/200 - 1/1000. WB: Use at a dilution of 1/200 - 1/1000. Detects a band of approximately 21.6 - 26.3 kDa. Preparation InstructionsMethod 1 for immunostaining and immunoblotting (Western blot): Remove a fixed amount of suspension and dissolve 1/5 in PBS or TBS to yield a 0.1 mg/ml solution. Method 2 for immunoprecipitation, supershift, immunostaining and immunoblotting (Western blot): Pellet antibodies. Remove supernatant. Dissolve the pellet in 50ul of PBS (or TBS) at final concentration of 1 mg/ml. Do not allow the pellet to dry. Do not vortex. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at −20°C |
| Storage Buffer | Preservative: NoneConstituents: 50% Ammonium sulphate, PBS |
