Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: A549 15% SDS PAGE diluted at 1:1000| Product Name | nm23-H2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to nm23-H2 (non-metastatic cells 2, protein (NM23B) expressed in)Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human nm23-H2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, BovineRat (98%), Xenopus laevis (87%), Dog (92%), Pig (93%), Rabbit (98%), Chicken (92%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC111212 antibody MGC2212 antibody NDKB antibody NDPK-B antibody NDPKB antibody NM23-H2 antibody NM23B antibody PUF antibody NME2 antibody NDP kinase B antibody "non-metastatic cells 2 protein (NM23) expressed in antibody" nucleotide diphospha |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) (5) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: A549 15% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
