Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDAR phosphorylated at Tyr1325 (Control) using NDMAR2A (phospho Tyr1325) antibody . The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).| Product Name | NMDAR2A (phospho Tyr1325) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NMDA NR2A Subunit (phospho Tyr1325)NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 conjugated to KLH |
| Modification | p-Tyr1325 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | glutamate receptor ionotropic Nmethyl Daspartate 2A NMDAR2A GRIN2A glutamate receptor ionotropic Nmethyl Daspartate2A NR2A glutamate receptor ionotropic N-methyl D-aspartate 2A NDMAR2A (phospho Y1325) NDMAR2A (phospho Tyr1325) NDMAR2A (pY1325 |
|---|---|
| Target | NMDAR2A (phospho Tyr1325) |
| Molecular Weight(MW) | 180 (note) |
Application
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDAR phosphorylated at Tyr1325 (Control) using NDMAR2A (phospho Tyr1325) antibody . The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
