Western blot analysis of extracts from A549 tissue using n-NOS (Phospho-Ser852) antibody. The lane on the right is treated with the antigen-specific peptide.
Rabbit Polyclonal antibody to nNOS (phospho Ser852)The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]
Immunogen
Peptide sequence around phosphorylation site of serine 852(F-N-S(p)-V-S) derived from Human n-NOS .
Modification
p-Ser852
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
The antibody detects endogenous level of n-NOS only when phosphorylated at serine 852.
Application
Application
Western blot analysis of extracts from A549 tissue using n-NOS (Phospho-Ser852) antibody. The lane on the right is treated with the antigen-specific peptide.
Application Notes
WB: 1:500 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20˚C/1 year
Storage Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.