Application
Immunofluorescence staining of methanol-fixed Hela cells using nNOS(Ab-852) AntibodyApplication
Western blot analysis of extracts from Rat and Mouse brain tissue using nNOS(Ab-852) Antibody| Product Name | nNOS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to nNOSProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. |
| Immunogen | Peptide sequence around aa.850~854 (F-N-S-V-S) derived from Human nNOS. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | IHPS1 N-NOS NC-NOS NOS bNOS nNOS NOS1 NOS type I constitutive NOS peptidyl-cysteine S-nitrosylase NOS1 nitric oxide synthase brain neuronal NOS nitric oxide synthase 1 (neuronal) |
|---|---|
| Target | nNOS |
| Molecular Weight(MW) | 160 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using nNOS(Ab-852) Antibody
Application
Western blot analysis of extracts from Rat and Mouse brain tissue using nNOS(Ab-852) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
