Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NOD1 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Product Name | NOD1 antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NOD1 (nucleotide-binding oligomerization domain containing 1)This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NOD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (81%), Rat (82%), Pig (87%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CARD4 antibody, CLR7.1 antibody, NLRC1 antibody, NOD1 antibody, "NLR family, CARD domain containing 1 antibody", "caspase recruitment domain family, member 4 antibody", caspase recruitment domain-containing protein 4 antibody, nucleotide-binding oligomeriz |
|---|---|
| Target | NOD1 |
| Molecular Weight(MW) | 108 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NOD1 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
