Application
NPAS1 antibody detects NPAS1 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg IMR32 whole cell lysate/extract C. 30 µg SK-N-AS whole cell lysate/extract 7.5 % SDS-PAGE NPAS1 antibody dilution: 1:1000Application
NPAS1 antibody detects NPAS1 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: NPAS1 protein stained by NPAS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
NPAS1 antibody detects NPAS1 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NPAS1 antibody dilution: 1:500.Application
NPAS1 antibody detects NPAS1 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. NPAS1 antibody dilution: 1:500.Application
NPAS1 antibody detects NPAS1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. NPAS1 antibody dilution: 1:500.| Product Name | NPAS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NPAS1 (neuronal PAS domain protein 1)The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. Studies of a related mouse gene suggest that it functions in neurons. The exact function of this gene is unclear, but it may play protective or modulatory roles during late embryogenesis and postnatal development. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NPAS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | bHLHe11 MOP5 PASD5 neuronal PAS domain protein 1 |
|---|---|
| Target | NPAS1 |
| Cellular Localization | Nucleus |
Application
NPAS1 antibody detects NPAS1 protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract B. 30 µg IMR32 whole cell lysate/extract C. 30 µg SK-N-AS whole cell lysate/extract 7.5 % SDS-PAGE NPAS1 antibody dilution: 1:1000
Application
NPAS1 antibody detects NPAS1 protein at nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: NPAS1 protein stained by NPAS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
NPAS1 antibody detects NPAS1 protein at nucleus on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. NPAS1 antibody dilution: 1:500.
Application
NPAS1 antibody detects NPAS1 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. NPAS1 antibody dilution: 1:500.
Application
NPAS1 antibody detects NPAS1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. NPAS1 antibody dilution: 1:500.| Positive Control | U87-MG , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
