Application
Western blot analysis of NPC1 in human kidney tissue lysate with NPC1 antibody at 1 ug/mL.Application
Immunohistochemistry of NPC1 in mouse kidney tissue with NPC1 antibody at 2.5 ug/mL.Application
Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 antibody at 20 ug/mL.| Product Name | NPC1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NPC1This gene encodes a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. It is predicted to have a cytoplasmic C-terminus, 13 transmembrane domains, and 3 large loops in the lumen of the endosome - the last loop being at the N-terminus. This protein transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. Defects in this gene cause Niemann-Pick type C disease, a rare autosomal recessive neurodegenerative disorder characterized by over accumulation of cholesterol and glycosphingolipids in late endosomal/lysosomal compartments.[provided by RefSeq, Aug 2009] |
| Immunogen | NPC1 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human NPC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 ug/ml. IHC-P: 2.5 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NPC Niemann-Pick disease type C1 |
|---|---|
| Target | NPC1 |
Application
Western blot analysis of NPC1 in human kidney tissue lysate with NPC1 antibody at 1 ug/mL.
Application
Immunohistochemistry of NPC1 in mouse kidney tissue with NPC1 antibody at 2.5 ug/mL.
Application
Immunofluorescence of NPC1 in Mouse Kidney cells with NPC1 antibody at 20 ug/mL.| Application Notes | WB: 1 ug/ml. IHC-P: 2.5 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
