Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NR0B2 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using NR0B2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using NR0B2 antibody at 1:100 dilution.| Product Name | NR0B2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NR0B2 (nuclear receptor subfamily 0, group B, member 2)The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NR0B2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (80%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ17090 antibody SHP antibody SHP1 antibody NR0B2 antibody orphan nuclear receptor SHP antibody small heterodimer partner antibody nuclear receptor SHP antibody nuclear receptor subfamily 0 group B member 2 antibody "nuclear receptor subfamily 0 |
|---|---|
| Molecular Weight(MW) | 28 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NR0B2 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using NR0B2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using NR0B2 antibody at 1:100 dilution.| Positive Control | 293T transfected with NR0B2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
