Application
NSE antibody detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
NSE antibody detects NSE protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NSE antibody diluted at 1:500.Application
NSE antibody detects NSE protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NSE antibody diluted at 1:500.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody diluted at 1:5000.| Product Name | NSE antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NSE (enolase 2, gamma neuronal)Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse NSE. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Chicken, Rhesus Monkey, BovineHuman (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AI837106 antibody D6Ertd375e antibody Eno2 antibody NSE antibody NSE antibody enolase 2 gamma neuronal antibody |
|---|---|
| Target | NSE |
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Cytoplasm (By similarity) , Cell membrane (By similarity) |
Application
NSE antibody detects NSE protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: NSE protein stained by NSE antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
NSE antibody detects NSE protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NSE antibody diluted at 1:500.
Application
NSE antibody detects NSE protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. NSE antibody diluted at 1:500.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with NSE antibody diluted at 1:5000.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
