Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using NT5C2 antibody at 1:100 dilution.| Product Name | NT5C2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NT5C2 (5'-nucleotidase, cytosolic II)Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human NT5C2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (100%), Xenopus laevis (87%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GMP antibody NT5B antibody PNT5 antibody cN-II antibody NT5C2 antibody cytosolic 5'-nucleotidase II antibody "5'-nucleotidase (purine) cytosolic type B antibody" IMP-specific 5'-NT antibody purine 5' nucleotidase antibody cytosolic purine 5'-nucl |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using NT5C2 antibody at 1:100 dilution.| Positive Control | HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
