Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:500Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NT5C2 antibody at 1:100 dilution.Application
Sample(30 ug whole cell lysate) A:H1299 B:HeLa S3 7.5% SDS PAGE diluted at 1:1000| Product Name | NT5C2 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NT5C2 (5'-nucleotidase, cytosolic II)Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NT5C2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Chicken, Rhesus Monkey, BovineXenopus laevis (93%), Chicken (96%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GMP antibody NT5B antibody PNT5 antibody cN-II antibody NT5C2 antibody cytosolic 5'-nucleotidase II antibody "5'-nucleotidase (purine) cytosolic type B antibody" IMP-specific 5'-NT antibody purine 5' nucleotidase antibody cytosolic purine 5'-nucl |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 7.5% SDS PAGE diluted at 1:500
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using NT5C2 antibody at 1:100 dilution.
Application
Sample(30 ug whole cell lysate) A:H1299 B:HeLa S3 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
