Application
IHC-P analysis of human kidney tissue using NCL antibody at 4.0-8.0μg/ml.Application
IHC-P analysis of human lung tissue using NCL antibody at 4.0-8.0μg/ml.Application
WB analysis of Jurkat cells using NCL antibody at 0.2-1μg/ml.| Product Name | Nucleolin antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NucleolinNucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to a N-terminal region of Human NCL |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Cow: 87%; Dog: 93%; Horse: 93%; Human: 100%; Mouse: 91%; Pig: 86%; Rabbit: 77%; Rat: 93%; Yeast: 83%; Zebrafish: 85% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NCL C23 |
|---|---|
| Target | NCL |
| Molecular Weight(MW) | 77 KDa (note) |
Application
IHC-P analysis of human kidney tissue using NCL antibody at 4.0-8.0μg/ml.
Application
IHC-P analysis of human lung tissue using NCL antibody at 4.0-8.0μg/ml.
Application
WB analysis of Jurkat cells using NCL antibody at 0.2-1μg/ml.| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
