Application
Western blot analysis of extracts from Mouse heart tissue using NPM (Phospho-Thr199) Antibody. The lane on the left is treated with the antigen-specific peptide.| Product Name | Nucleophosmin (phospho Thr199) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleophosmin (phospho Thr199)This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009] |
| Immunogen | Peptide sequence around phosphorylation site of Threonine 199 (R-D-T(p)-P-A) derived from Human NPM. |
| Modification | p-Thr199 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nucleophosmin (nucleolar phosphoprotein B23 numatrin) NPM B23 NPM1 |
|---|---|
| Target | Nucleophosmin (phospho Thr199) |
| Tissue Specificity | The antibody detects endogenous level of NPM only when phosphorylated at Threonine 199. |
Application
Western blot analysis of extracts from Mouse heart tissue using NPM (Phospho-Thr199) Antibody. The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
