Application
Western blot analysis of Nucleophosmin (pT234) expression in MCF7 colchicine-treated (A); LNCaP (B); HeLa (C); Raw264.7 colchicine-treated (D); PC12 colchicine-treated (E) whole cell lysates.| Product Name | Nucleophosmin (phospho Thr234) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleophosmin (phospho Thr234)This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Nucleophosmin. The exact sequence is proprietary. |
| Modification | p-Thr234 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NPM B23 NPM1 nucleophosmin (nucleolar phosphoprotein B23 numatrin) |
|---|---|
| Target | Nucleophosmin (phospho Thr234) |
| Tissue Specificity | Recognizes endogenous levels of Nucleophosmin (pT234) protein. |
Application
Western blot analysis of Nucleophosmin (pT234) expression in MCF7 colchicine-treated (A); LNCaP (B); HeLa (C); Raw264.7 colchicine-treated (D); PC12 colchicine-treated (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
