Application
Nucleophosmin antibody detects Nucleophosmin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nucleophosmin antibody diluted at a dilution of 1:1000.Application
Nucleophosmin antibody detects Nucleophosmin protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleophosmin protein stained by Nucleophosmin antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nucleophosmin antibody dilution: 1:500.Application
Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Nucleophosmin antibody dilution: 1:500.| Product Name | Nucleophosmin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleophosmin (nucleophosmin (nucleolar phosphoprotein B23, numatrin))NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM] |
| Immunogen | Full length human Nucleophosmin Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rabbit, Chimpanzee, BovineMouse (92%), Rat (91%), Dog (97%), Rabbit (96%), Chimpanzee (99%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | B23 antibody MGC104254 antibody NPM antibody NPM1 antibody nucleolar protein NO38 antibody numatrin antibody "nucleophosmin/nucleoplasmin family member 1 antibody" nucleophosmin antibody "nucleophosmin (nucleolar phosphoprotein B23 numatrin) anti |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) |
| Cellular Localization | Nucleus , nucleolus |
Application
Nucleophosmin antibody detects Nucleophosmin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Nucleophosmin antibody diluted at a dilution of 1:1000.
Application
Nucleophosmin antibody detects Nucleophosmin protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Nucleophosmin protein stained by Nucleophosmin antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Nucleophosmin antibody dilution: 1:500.
Application
Nucleophosmin antibody detects Nucleophosmin protein at nucleus on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Nucleophosmin antibody dilution: 1:500.| Positive Control | 293T , A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
