Application
B. Immunohistochemical staining of paraffin-embedded non-Hodgkin?s lymphoma using anti-NPM RabMAb (cat. #).Application
A. Western blot analysis on HeLa cell lysate using anti-NPM RabMAb (cat. # ) dilution 1:200000.| Product Name | Nucleophosmin antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP1848Y] to NPM (N-term)Nucleophosmin (NPM), a nucleocytoplasmic shuttling protein with prominent nucleolar localization, regulates the ARF-p53 tumor-suppressor pathway. Translocations involving the NPM gene cause cytoplasmic dislocation of the NPM protein . NPM is a major nucleolar protein which is 20 times more abundant in tumor or proliferating cells than in normal resting cells. Data suggest that transcription factor YY1 may play a role in NPM gene expression . A chromosomal translocation associated with myelodysplastic syndrome and acute myeloid leukemia (AML) was found to rearrange part of the nucleophosmin (NPM) gene. Myeloid leukemia factor 1 (MLF1) binds NPM and studies have indicated that MLF1 is normally located in the cytoplasm, whereas NPM-MLF1 is targeted to the nucleus, with highest levels in the nucleolus. The nuclear/nucleolar localization of NPM-MLF1 mirrors that of NPM, indicating that NPM trafficking signals direct MLF1 to an inappropriate cellular compartment in myeloid leukemia cells |
| Immunogen | A synthetic peptide corresponding to residues on the N-terminus of human NPM was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1848Y |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PIPWB |
| For WB: Use at a dilution of 1:200,000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MGC104254 NPM B23 NPM1 |
|---|---|
| Target | NPM (N-term) |
| Molecular Weight(MW) | 35/38 (2 isoforms) (note) |
Application
B. Immunohistochemical staining of paraffin-embedded non-Hodgkin?s lymphoma using anti-NPM RabMAb (cat. #).
Application
A. Western blot analysis on HeLa cell lysate using anti-NPM RabMAb (cat. # ) dilution 1:200000.| Application Notes | For WB: Use at a dilution of 1:200,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
