Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NUBPL antibody diluted at 1:1000.Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NUBPL antibody diluted at 1:500.| Product Name | Nucleotide binding protein like antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Nucleotide binding protein like (nucleotide binding protein-like)Respiratory complex I (NADH:ubiquinone oxidoreductase EC 1.6.5.3) is a large mitochondrial inner membrane enzyme consisting of 45 subunits and 8 iron-sulfur (Fe/S) clusters. NUBPL, or IND1, is an Fe/S protein that plays a critical role in the assembly of respiratory complex I, likely by transferring Fe/S into the Fe/S-containing complex I subunits (Sheftel et al., 2009 [PubMed 19752196]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Nucleotide binding protein like. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (91%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NUBPL huInd1 C14orf127 nucleotide binding protein-like IND1 |
|---|---|
| Target | Nucleotide binding protein like |
| Molecular Weight(MW) | 34 kDa (note) |
Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NUBPL antibody diluted at 1:1000.
Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with NUBPL antibody diluted at 1:500.| Positive Control | HepG2, mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
