Application
Immunofluorescence analysis of methanol-fixed A431, using OAT antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using OAT antibody at 1:100 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | OAT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OAT (ornithine aminotransferase)This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme is the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human OAT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineRat (93%), Zebrafish (81%), Xenopus laevis (84%), Pig (94%), Chicken (86%), Rhesus Monkey (97%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp781A11155 antibody GACR antibody HOGA antibody OATASE antibody OKT antibody OAT antibody ornithine delta-aminotransferase antibody "ornithine aminotransferase mitochondrial antibody" ornithine--oxo-acid aminotransferase antibody ornithine-ox |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (7) |
| Cellular Localization | Mitochondrion matrix |
Application
Immunofluorescence analysis of methanol-fixed A431, using OAT antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using OAT antibody at 1:100 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HepG2 , Molt-4 , Raji , mouse brain , *K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
