Application
OCT1 antibody immunoprecipitates OCT1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of OCT1 by 2.5 μg of OCT1 antibody 7.5% SDS-PAGE The immunoprecipitated OCT1 protein was detected by OCT1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
OCT1 antibody detects OCT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT1 protein stained by OCT1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
OCT1 antibody detects OCT1 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. OCT1 antibody diluted at 1:500.Application
Immunoprecipitation of OCT1 protein from 293T whole cell extracts using 5 μg of OCT1 antibody . Western blot analysis was performed using OCT1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using OCT1 antibody at 1:750 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000| Product Name | OCT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to OCT1 (POU domain, class 2, transcription factor 1)The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human OCT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Pig, Chicken, Chimpanzee, BovineMouse (90%), Xenopus laevis (81%), Pig (97%), Chicken (91%), Chimpanzee (99%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ42836 antibody OTF1 antibody POU2F1 antibody oct-1 antibody NF-A1 antibody "POU domain class 2 transcription factor 1 antibody" octamer-binding protein 1 antibody octamer-binding transcription factor 1 antibody OTF-1 antibody Octamer-binding |
|---|---|
| Molecular Weight(MW) | 76 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
OCT1 antibody immunoprecipitates OCT1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 μg of pre-immune rabbit IgG B : Immunoprecipitation of OCT1 by 2.5 μg of OCT1 antibody 7.5% SDS-PAGE The immunoprecipitated OCT1 protein was detected by OCT1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
OCT1 antibody detects OCT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT1 protein stained by OCT1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
OCT1 antibody detects OCT1 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. OCT1 antibody diluted at 1:500.
Application
Immunoprecipitation of OCT1 protein from 293T whole cell extracts using 5 μg of OCT1 antibody . Western blot analysis was performed using OCT1 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using OCT1 antibody at 1:750 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
